traits argued that missing heritability can be explained by rare to low frequency variants of relatively large effect sizes. These rare variants can be identified by next generation sequencing in large cohorts. Contrary to expectations, recent sequencing studies for neurological disorders in moderate sample size are not much successful and have not yielded the intended results[63, 64]. These studies concluded that the low frequency disease associated variants generally have low-moderate effect sizes and very large sample size is needed to identify these variants. Sequencing costs are moving down, but still these costs are prohibitive to perform a large whole genome sequencing study of AUD and other complex psychiatric disorders.
