RPGMs were calculated from the three different GABAB1 gene boundaries: 1) known GABAB1 gene boundary from the RefSeq Genes model, 2) minimum GABAB1 gene boundary from RNA-seq, and 3) GABAB1 gene boundary from UCSC genome browser databases (Figure 5). However, the expression levels of these three gene boundaries did not significantly change in alcoholics. To confirm GABAB1 gene expression levels, RPGM values were calculated for all genes from the RefSeq Genes model and analyzed using DEGseq (21). As shown in Figure 5A, GABAB1 gene expression did not change significantly.
