genes for this manuscript) were found to be expressed in at least one structure (91.4% overlap in expressed gene sets between brains), consistent with the percentage of genes expressed in mouse brain by ISH (80%; ref. 1) and fetal human brain by microarrays (76%; ref. 11). Expression levels across anatomical structures were strongly correlated between brains (Pearson r = 0.98, P < 10−40), with a highly significant correlation in differential expression relationships between structures (Pearson r = 0.46, P < 10−40). Later in our analysis we completed data generation from a single (left) hemisphere of a third specimen. We found strong corroboration of overall expression levels and fold changes between structures in all three brains (Supplementary Fig. 2).
