4B and Fig. S8C). Although the CoREST complex consists of many proteins, the interaction between Nurr1 and CoREST seemed to be direct, as indicated by in vitro GST-pull down assay (Fig. S8D). This interaction was mediated by the DNA-binding domain of Nurr1 (Nurr1-DBD) (Fig. S8E). When Nurr1-DBD was overexpressed in Hela cells, the interaction between Nurr1 and CoREST was inhibited in a dose-dependent manner (Fig. S8F). Furthermore, overexpression of the Nurr1-DBD in RAW264.7 cells altered Nurr1-mediated repression of iNOS-promoter activity (Fig. S8G).
