altered expression of w (for example, >75% of the ~46,000 stocks in the Drosophila Bloomington Stock Center contain an allele of w or mini-w at the time of manuscript preparation), our findings represent a significant limitation to the utility of the eRING assay for the genetic analysis of ethanol sensitivity in Drosophila. A more detailed analysis that addresses possible mechanisms for the effect of mini-w and endogenous w on ethanol sensitivity in eRING studies will be presented elsewhere.