The master regulators refer to transcriptional factors determining the cell fate by dictating transcriptome, which has been well documented during reprogramming process. Extensive activation and inactivation of gene expression also occur in programmed differentiation1. Oligodendrocytes accelerate the conduction of nerve impulse by myelinating neuronal axons in the central nervous system (CNS)2,3. Dysregulated myelination is associated with various devastating neurological diseases, such as multiple sclerosis and leukodystrophy4–6. OPCs differentiate into iOLs that subsequently develop into mature oligodendrocytes (mOLs). Previous work has well painted the gene activation networks that promotes OPCs differentiation7,8. However, the importance and mechanism of gene downregulation in each stage for myelinogenes is were not fully understood.
