possible while ensuring homogenous droplet sizes. Oil flow rates were between 13–15 mL/hr. Cell suspensions were split between two Drop-seq rigs to reduce runtime. Drop-seq encapsulation was performed within 2 hours after suspension preparation. Drop-seq conditions for nuclei paralleled those outlined for cells (λnuclei ~ 0.08). The molecular workflow for Reverse Transcription, cDNA Amplification, Tagmentation and Sequencing follows that of Macosko et al. Note, the number of beads and corresponding STAMPs that were pooled for SMRT cDNA amplification varied between 2–8K beads/reaction.
