Consistent with previous results, we find the PGM introduces coverage bias against low and high G+C% sequences, with evidence suggesting that attempts to sequence high G+C% organisms (greater than 65% G+C%) may even fail during the PGM library preparation. Although the mechanisms behind these failed libraries are not evaluated here, we anticipate they will bias representation of high G+C% organisms in metagenomes and metatranscriptomes.
