In a study conducted in postmortem hippocampal tissue samples from alcoholics, reverse transcribed RNA and genomic DNA fragments immunoprecipitated with an antibody against histone H3 trimethylated at lysine 4 (H3K4me3, a marker of active transcription) were subjected to high-throughput sequencing (RNA-seq) and there was no overlap between loci of H3K4me3 and gene expression changes [42]. This suggests that histone H3 lysine 4 trimethylation does not play a critical role in mediating transcriptional response to chronic ethanol abuse in the hippocampus.
