Finally, BioAge and Inflame are consistent with published analysis of the healthy brain transcriptome and are associated with neuronal, astrocytic, and microglial modules [24]. Importantly, we found that NdStress and Inflame have virtually identical scores in different regions from the same individual. It suggests they measure systemic changes in brain tissue that happen across multiple cell types and layers and are independent of the diverse morphology and makeup of different brain regions. Alz scores, on the other hand, are not the same across all brain regions and had the highest levels in prefrontal cortex, indicating a local rather than systemic nature of EMT.
