We scanned labeled sections using a Hamamatsu NanoZoomer S360 or a Nikon Eclipse Ti2 under 20x objective. For high resolution images, we used a Nikon Eclipse Ti2 or an Olympus IX83 under 40x or 63x objective or 40x with additional 2x built in objective (equal to 80x). We took multi-layer images and did deconvolution using Nikon's NIS-Elements deconvolution software and used maximum intensity projections to create single images. We used NDP software to convert the NanoZoomer original files to tiff format and ImageJ and Adobe Photoshop to adjust brightness and overlay images.
