Toward this aim, the first test to perform after finding a genetic variant associated with a phenotype is to assess the variant activity predictions, using the already mentioned C-score and VEP analysis tool, and others such as PredictSNP2 (https://loschmidt.chemi.muni.cz/predictsnp2/)116. They are able to visualize, annotate and prioritize such data to guide the analyst toward a more focussed work hypothesis. However, if they do not make concrete predictions about the role of the polymorphisms, there are many additional databases to consult. For example, if a polymorphism changes the DNA sequence necessary for the binding of a transcription factor (TF), this can be tested by searching in TF databases,117 but it gives a probabilistic answer that will likely require further tests.
