RNA sequencing was performed as described recently16. The total RNA was extracted using mirVana™ miRNA Isolation Kit, with phenol (#AM1560, Thermo Fisher Scientific). RNA samples were DNAse-treated with DNA-free kit (#AM1906, Thermo Fisher Scientific), and ribosomal RNA was depleted using RiboMinus Eukaryote kit (Life Technologies). Two hundred and forty samples (30 alcoholics and 30 controls for each brain region) were processed using the TruSeq RNA Library Prep Kit v.2 and sequenced on the Illumina HiSeq 2 000 at the Genome Sequencing and Analysis Facility at The University of Texas at Austin. Paired-end libraries with an average insert size of 180 bp were obtained. Sequence read archives have submitted for all brain regions, and their accession numbers are as follows: PRJNA530758 (SFC), PRJNA551775 (NA), PRJNA551909 (BLA), and PRJNA551908 (CNA).
