To determine if prolonged antibiotic treatment caused the absorption of antibiotics that are not absorbed in normal animals, we treated animals for two weeks with the triple antibiotic cocktail via their drinking water as described above. Serum was collected as above. Serum samples from 24 mice were collected to form 8 replicates which were sent for testing in the Mount Sinai Hospital clinical laboratory. There they were assayed according to their standard vancomycin immunodetection assay according to the same protocols used to monitor therapeutic vancomycin concentrations in patients. The assay has a lower limit of detection of 1.1 μg/ml.
