In agreement with this conclusion, DEGs reported in ref. 10 distinguished our hESC and hiPSC cell lines by genetic background rather than cellular origin (Fig. 5D, left panel). In that study, multiple hiPSC lines generated from one man were compared to male and female hESC lines of different genetic backgrounds. Conversely, genes that distinguish our HUES2-derived and HUES3-derived pluripotent cell lines were able to separate the hESCs and hiPSCs in ref. 10 (Fig. 5D, right panel; Fig. 5E; Supplementary Fig. 4J,K).
