Microarray findings were verified with Nanostring Technologies (Nanostring, Seattle, WA) using 100ng NAc RNA from human heroin (N=20) and control (N=16) subjects. Design and synthesis of probes was performed according to company protocol and hybridization reactions were carried out as previously described.(41) Expression values were normalized to ACTB and GAPDH, which were stable in the human population. Probe sequences are listed in supplementary table 2. Statistical analyses were performed as above using JMP software.
