Chunk #13 — MATERIALS AND METHODS — DNA Resequencing in the CHRNA5 and CHRNA3 intergenic region

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The CHRNA5-CHRNA3-CHRNB4 nicotinic receptor subunit gene cluster affects risk for nicotine dependence in African-Americans and in European-Americans.

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Sequencing was performed at the Genome Center at Washington University using standard protocols3. We used Sequencher (Gene Codes, Ann Arbor, Michigan) to assemble and scan sequence traces for variation from the reference sequence. Novel variants were genotyped using Sequenom MassArray iPLEX technology.