To further validate our PAC results, we generated simulated data with known alpha-gamma coupling (10–11 Hz phase, 50–70 Hz amplitude). Using the same MATLAB code as for the MEG data, we were able to successfully detect this alpha-gamma PAC using the MVL-MI-Canolty, MVL-MI-Özkurt, PLV-MI-Cohen and KL-MI-Tort algorithms (Figure 5A). By varying the trial length of the simulated data, we found that PAC values were affected by trial length, with data segments under 1 s producing artificially inflated PAC (Figure 5B).
