Weight gains in both HFD-WT and HFD-Cyp2e1-null were significantly greater than their corresponding LFD-fed groups (P < 0.05) (Supplementary Fig. 1A). However, the ratios of liver to total body weight (liver index) were not significantly increased (Supplementary Fig. 1B) in any of the four groups despite the fact that histological analysis revealed that livers from both HFD-fed WT and Cyp2e1-null developed massive steatosis (Fig. 1). Plasma ALT and AST activities did not differ between WT and Cyp2e1-null groups regardless of different diets (Supplementary Fig. 1C), as previously described [10,11].
