Majority of the BXD and LXS tissues (cerebellum, eye, forebrain, hippocampus, kidney, liver, and striatum for the HQF Illumina dataset) were dissected at the University of Tennessee Health Science Center (UTHSC). Mice were housed at the UTHSC in pathogen-free colonies, at an average of three mice per cage. All animal procedures were approved by the Animal Care and Use Committee. Mice were killed by cervical dislocation, and tissues were rapidly dissected and placed in RNAlater (Ambion, www.ambion.com) and kept overnight at 4° C, and subsequently stored at −80 degree C. Tissue were then processed at UTHSC or shipped to other locations for processing.
