ATAC-seq data sets can also be bioinformatically separated into reads that are shorter than the canonical length generally protected by a nucleosome and reads consistent with the approximate length of DNA protected by a nucleosome. These different populations of reads provide information about the positions of nucleosomes, as well as nucleosome-free regions. In this way, paired-end ATAC-seq data provides MNase-seq-like data sets for nucleosomes at relatively accessible regions of the genome.
