Finally, a link between CYP2E1 and TNF-α production has been described in vitro in a macrophage cell line with stable expression of CYP2E1 [24]. In this model, increased expression of CYP2E1 was accompanied by increased levels of CD14/TLR-4, NADPH oxidase, and H2O2. The higher production of hydrogen peroxide resulted in activation of the MAPKs ERK1/2 and p38, which stimulated TNF-α production via activation of NF-kB and stabilization of TNF-α mRNA, respectively [24].
