Hierarchical agglomerative clustering was performed on trans-eGenes (50% FDR) and cis-eGenes (5% FDR) using distance metric (1 − Spearman’s ρ) of Meta-Tissue effect sizes across all observed genes between tissue pairs. To supplement this analysis, we also performed multi-tissue analysis using 1) replication analysis (Extended Data Fig. 7); 2) hierarchical FDR control17 for both cis and trans analysis (Supplementary Information 8); and 3) an empirical Bayes approach18.
