Chunk #50 — Methods — Western blot

Source

Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells.

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The FANCA antibody (Cell Signalling, D1L2Z) was used at 1:1000 in 5% w/v BSA, 1× TBS, 0.1% Tween-20 at 4 °C with gentle shaking, overnight. The β-actin antibody (Abcam, 8227) was used at 1:3,000 in the same conditions. Swine anti-rabbit immunoglobulins HRP (Dako) was used as secondary antibody at 1:2,000 for 1 h at room temperature.