We tested pharmacological manipulation of interneuron migration in fused hSS-hCS (Extended Data Fig. 7e). We imaged the movement of Dlxi1/2b::eGFP cells before and after exposure to a CXCR4 receptor antagonist (AMD3100). This receptor is expressed in hSS (Extended Data Fig. 3l) and plays a key role in the migration of cortical interneurons21. AMD3100 treatment resulted in a significant reduction in saltation frequency, saltation length, speed when mobile (Extended Data Fig. 7f–h) and a change in the path directness (Extended Data Fig 7i, j; Supplementary Video 6).
