The analysis was performed using summary statistics from the CUD GWAS and summary statistics from GWAS of smoking intiation30 (excluding 23andMe data, 547,219 individuals, 11,733,344 variants).The conditional analysis was run using mtCOJO46 (implemented in GCTA75) using standard procedures. Variants selected for conditioning were genome-wide significant in the smoking GWAS (P < 5x10−8) and not in linkage disequilibrium (r2< 0.05) with a variant with a lower P-value (61 variants). One variant was removed due to evidence of pleiotropy (HEIDI-outlier threshold of P= 0.01).
