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Chunk #6 — Online Methods — Phasing de novo mutation in MAGEL2 (also see Supplementary Figure 2)

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Truncating mutations of MAGEL2 cause Prader-Willi phenotypes and autism.
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KAPA HiFi and Pfu PCR products were gel purified (GeneJET Gel Extraction Kit, Fermentas, Waltham, MA) and submitted to Elim Biopharmaceuticals (Hayward, CA) for Sanger sequencing.