For prolonged alcohol exposure, cells exposed to 25 mM alcohol were placed in a Billups-Rothenburg chamber with twice the alcohol concentration in the bottom of the chamber to saturate the chamber and maintain the alcohol concentration in the wells for 7 days, as described earlier by other groups (15). The alcohol concentration in each well was measured using the Analox Alcohol Analyzer (Analox Instruments). The concentrations were maintained to 25 mM ± 4.5 over the 7-day period (mM EtOH: Day 1: 24 ± 0.1; Day 2: 21.5 ± 0.5; Day 3: 25 ± 0.1; Day 4: 22 ± 0.2; Day 5: 24 ± 0.7; Day 6: 22 ± 0.1; Day 7: 19.5 ± 0.5).
