The univariate model constraining genetic, common environmental and unique environmental influences on age 14 alcohol use frequency to be equal across urban and rural settings did not provide a significant decrease in goodness of fit for males (χ2Δ = 2.81, 3 df, p = 0.42) or females (χ2Δ = 0.22, 3 df, p = 0.97). Thus, the magnitude of importance of genetic and environmental influences on adolescent alcohol use did not significantly differ between urban and rural municipalities for either sex.