Some alcohol-fed mice received daily subcutaneous injections of 15 mg/kg body weight Cenicriviroc (CVC; provided by Tobira Therapeutics, Delaware, USA) for 6 weeks (“prevention” cohort) while others received the same dose daily for 3 weeks (beginning after 3 weeks of alcohol feeding and continued to week 6 of alcohol feeding; “treatment” cohort). Ten percent hydroxypropyl-β-cyclodextrin (Sigma, St. Louis, MO USA), 5% Kolliphor (solutol) HS15 (Sigma), and 85% sterile water were used as a vehicle to dissolve CVC into a solution. Pair-fed mice and alcohol-fed mice received daily injections of the vehicle without CVC for 6 weeks.
