Recent studies have applied iPSC technology to the study of BPD. Chen et al [21] generated iPSCs from 3 unrelated patients with Type 1 BPD and saw differential gene regulation in neurons derived from these cell lines when compared to neurons from lines derived from unaffected, unrelated controls. Madison et al [22] generated iPSCs from 2 affected and 2 unaffected individuals in a pedigree enriched for BPD. Comparison of gene expression profiles between BPD and control NPs and BPD and control neurons identified differentially regulated genes. To gain further insight into BPD, we chose to generate iPSCs and their derivatives from individuals within a 5 generation Old Order Amish pedigree with high prevalence of BPD [8]. We reasoned that transcriptomic analysis of iPSC-derived neurons from affected and unaffected first-degree relatives might not only offer a better approach to gain insights into the molecular changes of BPD by reducing both genetic and non- genetic variability, but moreover, might leverage the existing genetic data from this pedigree. The iPSC lines from 4 affected and 4 unaffected relatives were differentiated into neuroprogenitors (NPs)
