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Chunk #6 — MATERIALS AND METHODS — Ethanol exposure and RNA extraction

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Gene Expression Changes in Glutamate and GABA-A Receptors, Neuropeptides, Ion Channels, and Cholesterol Synthesis in the Periaqueductal Gray Following Binge-Like Alcohol Drinking by Adolescent Alcohol-Preferring (P) Rats.
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sessions (McBride et al., 2014). These levels of intake lead to BAC of 100 mg% (Bell et al., 2011), and therefore meet the criterion for binge-drinking put forth by the National Institute on Alcohol Abuse and Alcoholism (NIAAA, 2004). The rats were sacrificed at 49 days of age, 3 h after the 1st access session on their 15th day of drinking. All research protocols were approved by the Indiana University School of Medicine Institutional Animal Care and Use Committee and are in accordance with the guidelines of the Institutional Care and Use Committee of the National Institute on Drug Abuse, National Institutes of Health, and the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council 1996).