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Chunk #12 — Method — DNA Analyses

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Mineralocorticoid receptor Iso/Val (rs5522) genotype moderates the association between previous childhood emotional neglect and amygdala reactivity.
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Genomic DNA from all participants was isolated from buccal cells drawn from Oragene DNA self-collection kits (DNA Genotek, Inc., Kanata, Ont., Canada). Samples were genotyped using the Il-lumina Human 610-Quad BeadChip (see the online data supplement); rs5522 data were extracted based on our a priori hypotheses, and no other polymorphisms were tested. The average call rate was 94.7%. Mineralocorticoid receptor rs5522 did not differ significantly from Hardy-Weinberg equilibrium (p=0.83).