Glucose intolerance, associated with DIO, becomes more evident in C57BL/6j mice as obesity increases. In standard glucose tolerance tests (GTT), at 11 weeks, the HFD had little impact on the response. However, the blood glucose, measured in males, was decreased at all time-points in Cyp1b1-ko mice fed the LFD and HFD, relative to their WT counterparts, without significant decreases in the area under the curve (AUC) (Figure 3B). The AUC were, however, significantly decreased at this 11 week time point in our second study [38]. After 33 weeks, the glucose tolerance curves of the females on the LFD were similar to those of the 11 week males. Again, the female Cyp1b1-ko mice on the LFD consistently retained the lowered glucose levels (Figure 3C). This profile is indicative of increased insulin sensitivity in the Cyp1b1-ko mice. Female WT mice on the HFD for 33 weeks showed severe glucose intolerance, which was however, prevented in the Cyp1b1-ko mice (the AUC decreased by 30 percent (p=0.08) relative to the WT mice).
