Western blotting with [125I]-Protein-A detection: samples were subjected to SDS-PAGE and then immobilised on nitrocellulose by electrophoretic transfer. After washes with a TBS-based blocking buffer containing gelatin, the nitrocellulose was cut into strips and then incubated with primary antibodies. After washes, the [125I]-Protein-A was added to a final dilution of 1:1000. Following incubation on a rocking platform, the strips were again washed and then arranged for exposure to X-ray film.
