Based on the extensive linkage disequilibrium (LD) in GABRA2, results from previous studies were reviewed and a single SNP, rs279871, was selected for genotyping (Edenberg et al., 2004). Blood samples were analyzed at the Indiana University Alcohol Research Center. DNA was isolated using the “HotSHOT” method (Truett et al., 2000) where TaqMan probes are used for allelic discrimination (Applied BioSystems, Foster City, CA).
