After 9 weeks of differentiation, 3D neuronal spheroids were treated with BACE1 or γ-secretase inhibitor for two days and media was collected for Aβ 40 and 42 measurements by ELISA. Treating the cells for two consecutive days markedly decreased both Aβ 40 and 42 (Fig 9A and 9B), which was significant in all cases except for Aβ42 from the AD1 neurons treated with BACE1 inhibitor (Fig 9B). 3D neurons derived from subject AD1 did not exhibit reduced Aβ42 production in the presence of BACE1 inhibitor, unlike all of the remaining four lines (AD2-5) that exhibited significantly less Aβ42.
