For pharmacological manipulations, vigabatrin (Sigma, 25 µg/g body weight) (Wu et al., 2003), pentobarbital (25 µg/g body weight), rapamycin (LC Laboratories; 20 mg/kg body weight) (Kim et al., 2009) or corresponding vehicle control was intraperitoneally (i.p.) delivered once daily for a defined period of time as indicated. Maternal deprivation stress was carried out in a similar manner as previously described (Meaney et al., 1996). Different groups of pups were stereotaxically injected with retroviruses expressing shRNA-C1 or shRNA-D1 at P10 after the maternal separation procedures were finished. Tissue samples were processed and the same set of cellular phenotypes was analyzed in a double-blind manner.
