Cruz catalogue number sc-17320), mouse anti-Nestin monoclonal antibody (1:200, Chemicon catalogue number MAB5326), rabbit anti-TUJ1 polyclonal antibody (1:500, Covance catalogue number PRB-435P), chicken anti-MAP2 polyclonal antibody (1:1,000, Abcam catalogue number ab5392), rabbit anti-VGLUT1 polyclonal antibody (1:200, Synaptic Systems catalogue number ab5392), rabbit anti-GFP antibody (1:500, Invitrogen catalogue number A6455) and rabbit anti-GABA polyclonal antibody (1: 1,000, Sigma catalogue number A2052). The secondary antibodies (Jackson ImmunoResearch Laboratories) used were goat anti-chicken Alexa Fluor 647 (1:500, catalogue number 703-605-155), goat anti-rabbit CY3 (1:500, catalogue number 111-165-003), donkey anti-chicken Dylight 488 (1:500, catalogue number 703-485-155), donkey anti-rabbit CY3 (1:500, catalogue number 711-165-152), donkey anti-rabbit Alexa 488 (1:500, catalogue number 711-545-152), donkey anti-chicken Dylight 549 (1:500, catalogue number 703-505-155), donkey anti-goat Alexa 488 (1:500, catalogue number 705-545-147), donkey anti-mouse CY3 (1:500, catalogue number 715-165-151), donkey anti-goat CY3 (1:500, catalogue number 705-165-147) and donkey anti-mouse Alexa 488 (1:500, catalogue number 715-545-151). All relevant information about the antibodies used in this study, including citation, clone number and antibody validation profile, can be found at the manufacturers’ websites.
