We used partitioned LDSC to evaluate which somatic tissues were enriched for major depression heritability93. Gene expression data generated using mRNA-seq from multiple human tissues were obtained from GTEx v6p (URLs). Genes for which <4 samples had at least one read count per million were discarded, and samples with <100 genes with at least one read count per million were excluded. The data were normalized, and a t-statistic was obtained for each tissue by comparing the expression in each tissue with the expression of all other tissues with the exception of tissues related to the tissue of interest (e.g., brain cortex vs all other tissues excluding other brain samples), using sex and age as covariates. A t-statistic was also obtained for each tissue among its related tissue (ex: cortex vs all other brain tissues) to test which brain region was the most associated with major depression, also using sex and age as covariates. The top 10% of the genes with the most extreme t-statistic were defined as tissue specific. The coordinates for these genes were extended by a 100kb window
