GWE analysis was performed on monocytes RNA samples using the Illumina HT-12 v3 BeadChip (http://www.Illumina.com). RNA samples were processed in batches of 96 samples. Two hundred ng of total RNA was reverse transcribed, amplified and biotinylated using the Illumina TotalPrep-96 RNA Amplification Kit (Ambion/Applied Biosystems, Darmstadt, Germany). 700 ng of each biotinylated cRNA was hybridized to a single BeadChip at 58°C for 16–18 hours. BeadChips were scanned using the Illumina Bead Array Reader.
