for control AD-iNs in terms of average mIPSC frequency (Fig. 5C) and amplitude (Fig. 5D). Interestingly, CIE AD-iNs showed an opposite phenotype to that observed with acute ethanol application (Fig. 3). The frequency of mIPSCs was significantly increased in D40 AD-iNs compared to controls (Fig. 5C, ***p ≤ 0.001), with only a modest increase observed in N40 (Fig. 5C). In addition, there was a significant genotypic difference between N40 and D40 AD-iNs following CIE (Fig. 5C, # # #p ≤ 0.001). There were no changes observed in the amplitude of mIPSCs for both N40 and D40 AD-iNs (Fig. 5D), indicating a presynaptic effect for CIE application exclusively in D40 AD-iNs. These data suggest a differential sensitivity to ethanol exposure between MOR N40 and D40 AD-iNs, which is both concentration- and duration-dependent.
