While our SILAC data identified upregulation of the actin-binding proteins (PFN1, CFL1 and CFL2), which are direct targets of RHO/ROCK activity, the ROCK inhibitor Y27632 failed to ameliorate aberrant SZ migration, instead significantly inhibiting migration of both control (P<0.00001) and SZ (P<0.000001) neurospheres (128 total SZ neurospheres were analyzed relative to 112 total control neurospheres) (Supplementary Figure 6I).
