al, submitted) and are not discussed in this manuscript. Mice were killed by cervical dislocation four hours following IP injection. Immediately thereafter, brains were extracted and chilled for one minute in iced phosphate buffer before being microdissected into 8 constituent regions as described previously [21], including medial prefrontal cortex, nucleus accumbens and ventral midbrain, which includes ventral tegmental area and substantia nigra. Excised regions were placed in individual tubes, flash-frozen in liquid nitrogen and stored at −80°C.
