Chunk #31 — MATERIALS AND METHODS — NPC maintenance

Source

Early pathogenic event of Alzheimer's disease documented in iPSCs from patients with PSEN1 mutations.

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NPCs were cultured on Matrigel-coated dishes in the NPC medium consisting of DMEM/F12 and neurobasal medium (1:1), 0.5% N2, 1% B27, 1% GlutaMax-1, 1% NEAA, 50 μM β-ME, 1% P/S, 10 ng/ml bFGF. NPCs differentiated in the NPC medium without bFGF for spontaneous neural differentiation into neuronal or glial cells.