WT and KO mice (n = 4 per time point) were administered ethanol (2 g/kg, i.p.) acutely. At 15, 45 and 120 minutes following administration, mice were sacrificed by cervical dislocation and trunk blood collected into heparinised capillary tubes. Blood samples were centrifuged for 10 minutes and supernatant frozen at −80°C. Samples were analysed in triplicate using an Analox automatic ethanol analyser (AM1, Analox Instruments, London, UK) and the mean value taken for statistical analysis.
