To determine the consequences of these elevated anandamide levels for plasticity within amygdala circuits,54 we performed slice electrophysiological analysis of AM3506′s effects at the BLA synapses. LFS (100 stimuli at 1 Hz) of the external capsule has been shown to induce a form of endocannabinoid- and CB1R-mediated LTDi in the BLA.3,55,56 To test whether AM3506 enhanced this form of plasticity, 2 μm AM3506 or vehicle was applied to the amygdala slices. Monosynaptically eIPSCs were recorded from visually identified pyramidal neurons via stimulation by an electrode placed within the BLA just medial to the external capsule (Figure 2d). LTDi was induced by LFS at twice the stimulation intensity of a test pulse (paired pulses elicited at 0.1 Hz, with 100 ms inter-stimulus interval).
