sorted directly into Neurobasal media at room temperature using a 100 µm nozzle, 20 psi sheath pressure, 2,000 events/sec; taking care not to subject the cells to excessive shear forces that would reduce viability. Cells were plated with ROCK Inhibitor (10 ng/mL) at a concentration of 1–4×105 cells in one well of a six well plate or 0.5 – 0.9 ×105 cells in one well of a 12-well plate.
