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Chunk #28 — 3. Methods — 3.7. Immunohistochemistry after transplantation — 3.7.2. Immunohistochemistry and cell counting

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Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells.
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Four months after transplantation, some of grafted cells expressed more mature cortical interneuron markers Somato-statin, Parvalbumin, Calretinin, Neuropeptide Y and Calbindin (Fig. 6m – q and Table 5). Transplanted cells generated very small numbers of astrocytes (GFAP+; Fig. 6r and Table 5) or oligodendrocyte lineage cells at this time point (Olig2+; Fig. 6s and Table 5).