Third, CRISPRi with three gRNAs in HepG2 cells (G/G, major/major at rs10889356; used because being of hepatic origin they express ANGPTL3) both decreased DOCK7 expression and increased ANGPTL3 expression (Figure 4F). These data are concordant with the data from the genome-edited hPSC clones with respect to the direction of effects for both genes. Taken together, all of these findings support rs10889356-DOCK7 and rs10889356-ANGPTL3 as functional SNP-gene sets.
